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Detection of Y Chromosome Microdeletions by PCR

Y chromosome micro-deletions like DAZ (deletion in azoospermia) and RBM (RNA binding motif gene) cause spermatogenic defects. In the Yq arm, intervals V and VI comprise an Azoospermia Factor (AZF) which contains the DAZ and RBM genes. Several genes have been identified within this region and have been proposed candidates for infertility. We now check for 20 different micro-deletions in the AZF region by multiplex PCR.

Sample: 3-5ml Blood in Potassium EDTA vaccutainer (purple top tube), transported at room temperature.

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