Sperm FISH

Routine analysis of sperm chromosomes is not feasible in clinical practice. However, interphase FISH on spermatozoa offers an accurate and reliable method of analysis even in the presence of a low sperm count. The percentage of sperm with hypo/hyperhaploidy is calculated to counsel the couple regarding chances of a normal pregnancy.

Sample : Fresh semen sample.

Outstation samples are accepted after partial processing in any andrology laboratory. The ‘swim-up’ sperm and pellet are to be separated in 2 tubes and incubated in hypotonic KCl (0.075M) for 20 minutes, following which a few drops of Carnoys fixative are added. After centrifugation, fresh fixative is added to the pellets before transportation.

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