Routine analysis of sperm chromosomes is not feasible in clinical practice. However, interphase FISH on spermatozoa offers an accurate and reliable method of analysis even in the presence of a low sperm count.
The percentage of sperm with hypo/hyperhaploidy is calculated to counsel the couple regarding chances of a normal pregnancy.Sample : Fresh semen sample.
Outstation samples are accepted after partial processing in any andrology laboratory. The ‘swim-up’ sperm and pellet are to be separated in 2 tubes and incubated in hypotonic KCl (0.075M) for 20 minutes, following which a few drops of Carnoys fixative are added. After centrifugation, fresh fixative is added to the pellets before transportation.